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|New modular platform based on multi-adjuvanted amphiphilic chitosan nanoparticles for efficient lipopeptide vaccine delivery against group A streptococcus
|Abdin Shakirin Mohamad Norpi
Muhammad Luqman Nordin
Abdullah Al-Hadi Ahmad Fuaad
|Amphiphilic chitosan nanoparticles
Multi-adjuvanting delivery system
Group A streptococcus
|Shenyang Pharmaceutical University
|Abdin Shakirin Mohamad Norpi, Muhammad Luqman Nordin, Nuraziemah Ahmad, Haliza Katas, Abdullah Al-Hadi Ahmad Fuaad, Asif Sukri, Marasini, N. & Fazren Azmi. (2022). New modular platform based on multi-adjuvanted amphiphilic chitosan nanoparticles for efficient lipopeptide vaccine delivery against group A streptococcus. Asian Journal of Pharmaceutical Sciences, 17(3), 435–446. https://doi.org/10.1016/j.ajps.2022.04.002
|An effective vaccine against group A streptococcus (GAS) is highly desirable for definitive control of GAS infections. In the present study, two variants of amphiphilic chitosan nanoparticles-based GAS vaccines were developed. The vaccines were primarily composed of encapsulated KLH protein (a source of T helper cell epitopes) and lipidated M-protein derived B cell peptide epitope (lipoJ14) within the amphiphilic structure of nanoparticles. The only difference between them was one of the nanoparticles vaccines received additional surface coating with poly (I:C). The formulated vaccines exhibited nanosized particles within the range of 220–240 nm. Cellular uptake study showed that nanoparticles vaccine without additional poly (I:C) coating has greater uptake by dendritic cells and macrophages compared to nanoparticles vaccine that was functionalized with poly (I:C). Both vaccines were found to be safe in mice and showed negligible cytotoxicity against HEK293 cells. Upon immunization in mice, both nanoparticle vaccines produced high antigen-specific antibodies titres that were regulated by a balanced Th1 and Th2 response compared to physical mixture. These antibodies elicited high opsonic activity against the tested GAS strains. Overall, our data demonstrated that amphiphilic chitosan nanoparticles platform induced a potent immune response even without additional inclusion of poly (I:C).
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