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Title: Synthesis and characterization of mangiferin loaded n,o-cmc nanoparticles and its cytotoxic effect on osteosarcoma mg-63 cells
Authors: Puteri Zarith Sofea Yusri
Nurin Fatini Ghazali
Nurul Azima Mazlan
Lum, Pei Teng
Aina Akmal Mohd Noor
Mani, Shankar
Sekar, Mahendran
Keywords: 3T3 cells
MG-63 cells
N-O-CMC nanoparticles
Issue Date: Apr-2020
Publisher: J. K. Welfare and Pharmascope Foundation
Citation: Puteri Zarith Sofea Yusri, Nurin Fatini Ghazali, Nurul Azima Mazlan, Lum, P. T., Aina Akmal Mohd Noor, Mani, S., & Sekar, M. (2020). Synthesis and Characterization of Mangiferin Loaded N,O-CMC Nanoparticles and its Cytotoxic Effect on Osteosarcoma MG-63 Cells. International Journal of Research in Pharmaceutical Sciences, 11(2), 2136–2145.
Abstract: Mangiferin is a xanthone glycoside, naturally isolated from Mangifera indica. Mangiferin has been reported for a wide range of pharmacological activities and its anticancer potential is very well known. However, the mangiferin anti-cancer potency is inadequate due to its poor water solubility. N,O-Carboxymethyl Chitosan (N,O-CMC) is a smart biopolymer, in which its bio-compatible, biodegradable and non-toxic making it ideal for abundant biological applications include the delivery of lipid soluble drugs. Also use-ful to improve and replace biological tissues and gene therapy. Hence, this study attempts to synthesize and characterize mangiferin-N,O-CMC nanopar-ticles and evaluate its antioxidant and cytotoxic properties. The mangiferin-N,O-CMC nanoparticles were prepared by loading mangiferin into N,O-CMC nanoparticles and characterized by FT-IR, DLS, SEM, Zeta potential and XRD measurements. In-vitro antioxidant was carried out by the DPPH method. The cytotoxic effect of mangiferin-N,O-CMC nanoparticles was carried out on Osteosarcoma MG-63 and 3T3 cells by using MTT assay method. The synthesized mangiferin-N,O-CMC nanoparticles with particle size ranges from 200±10 nm. The charge of N,O-CMC nanoparticles were confirmed by Zeta potential and found to be −45.8 mV. In the DPPH method, mangiferin-N,O-CMC nanoparticles showed IC50 value between 7.8-15.6 µg/ml. In MTT assay, mangiferin-N,O-CMC nanoparticles exhibited a significant reduction in the growth of osteosarcoma MG-63 cells and there is no toxic effect against nor-mal 3T3 cells. These findings designated that the synthesized mangiferin-N,O-CMC nanoparticles were very efficient nanocarrier in delivering the mangiferin to cancer cells.
ISSN: 09757538
Appears in Collections:Journal Articles

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