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http://hdl.handle.net/123456789/29528
Title: | New modular platform based on multi-adjuvanted amphiphilic chitosan nanoparticles for efficient lipopeptide vaccine delivery against group A streptococcus |
Authors: | Abdin Shakirin Mohamad Norpi Muhammad Luqman Nordin Nuraziemah Ahmad Haliza Katas Abdullah Al-Hadi Ahmad Fuaad Asif Sukri Marasini, Nirmal Fazren Azmi (UniKL RCMP) |
Keywords: | Amphiphilic chitosan nanoparticles Peptide vaccine Lipidation Multi-adjuvanting delivery system Immunogenicity Group A streptococcus |
Issue Date: | May-2022 |
Publisher: | Shenyang Pharmaceutical University |
Citation: | Abdin Shakirin Mohamad Norpi, Muhammad Luqman Nordin, Nuraziemah Ahmad, Haliza Katas, Abdullah Al-Hadi Ahmad Fuaad, Asif Sukri, Marasini, N. & Fazren Azmi. (2022). New modular platform based on multi-adjuvanted amphiphilic chitosan nanoparticles for efficient lipopeptide vaccine delivery against group A streptococcus. Asian Journal of Pharmaceutical Sciences, 17(3), 435–446. https://doi.org/10.1016/j.ajps.2022.04.002 |
Abstract: | An effective vaccine against group A streptococcus (GAS) is highly desirable for definitive control of GAS infections. In the present study, two variants of amphiphilic chitosan nanoparticles-based GAS vaccines were developed. The vaccines were primarily composed of encapsulated KLH protein (a source of T helper cell epitopes) and lipidated M-protein derived B cell peptide epitope (lipoJ14) within the amphiphilic structure of nanoparticles. The only difference between them was one of the nanoparticles vaccines received additional surface coating with poly (I:C). The formulated vaccines exhibited nanosized particles within the range of 220–240 nm. Cellular uptake study showed that nanoparticles vaccine without additional poly (I:C) coating has greater uptake by dendritic cells and macrophages compared to nanoparticles vaccine that was functionalized with poly (I:C). Both vaccines were found to be safe in mice and showed negligible cytotoxicity against HEK293 cells. Upon immunization in mice, both nanoparticle vaccines produced high antigen-specific antibodies titres that were regulated by a balanced Th1 and Th2 response compared to physical mixture. These antibodies elicited high opsonic activity against the tested GAS strains. Overall, our data demonstrated that amphiphilic chitosan nanoparticles platform induced a potent immune response even without additional inclusion of poly (I:C). |
URI: | https://ir.unikl.edu.my/jspui/handle/123456789/29528 |
ISSN: | 18180876 |
Appears in Collections: | Journal Articles |
Files in This Item:
File | Description | Size | Format | |
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New modular platform based.pdf | 2.88 MB | Adobe PDF | View/Open |
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