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http://hdl.handle.net/123456789/26396
Title: | Occurrence of extended-spectrum β-lactamase, AmpC, and carbapenemase-producing genes in gram-negative bacterial isolates from human immunodeficiency virus infected patients |
Authors: | Rameshkumar, Marimuthu Ragavan Arunagirinathan, Narasingam Senthamilselvan, Balasubramanian Swathirajan, Chinnambedu Ravichandran Solomon, Sunil Suhas Vignesh, Ramachandran Balakrishnan, Pachamuthu Aljowaie, Reem M. Almaary, Khalid S. Chen, Tse-Wei (UniKL RCMP) |
Keywords: | blaCTX-M Extended-spectrum β-lactamases HIV patients Klebsiella pneumoniae Multidrug-resistant |
Issue Date: | Dec-2021 |
Publisher: | Elsevier Ltd |
Citation: | Rameshkumar, M. R., Arunagirinathan, N., Senthamilselvan, B., Swathirajan, C. R., Solomon, S. S., Vignesh, R., Balakrishnan, P., Aljowaie, R. M., Almaary, K. S., & Chen, T.-W. (2021). Occurrence of extended-spectrum β-lactamase, AmpC, and carbapenemase-producing genes in gram-negative bacterial isolates from human immunodeficiency virus infected patients. Journal of Infection and Public Health, 14(12), 1881–1886. https://doi.org/10.1016/j.jiph.2021.11.008 |
Abstract: | Background: Progressive decline of immune response in HIV patients makes them susceptible to frequent bacterial infections. High usage of antibiotics influences the emergence of multidrug-resistant bacteria and worsens the clinical outcomes. In this study, the occurrence of drug-resistant genes in Gram-negative bacterial isolates from HIV patients in South India was analyzed. Methods: A total of 173 Gram-negative bacterial (GNB) isolates from HIV patients were screened for antibiotic susceptibility profile using the Kirby-Bauer diskdiffusion method. Positivity of drug-resistant genes was analyzed using polymerase chain reaction method. Results: In this study, 72.8% of bacterial isolates were obtained from urine specimens, and Escherichia coli (47.4%) was the predominantly isolated bacterium. Overall, 87.3% and 83.2% of GNB were resistant to 3rd generation cephalosporin antibiotics such as cefotaxime and ceftazidime, respectively, 56.6% were resistant to cephamycin (cefoxitin) and 43% to carbapenem (imipenem) antibiotics. Extended-spectrum β-lactamases (ESBL) production was noted among 79.5% of GNB isolates, followed by AmpC (57.1%) and Metallo β-lactamases (37.3%). Molecular analysis revealed that ESBL genes such as blaTEM (94.1%), blaCTX-M (89.2%), and blaSHV (24.2%) were detected at higher levels among GNB isolates. Carbapenemase-producing genes such as blaOXA-48 (20%), blaOXA-23 (2.6%), and both blaOXA-23 and blaOXA-51 like genes (2.6%) and AmpC producing genes such as blaCIT (26.7%), blaDHA (3.6%), and blaACC (1.8%) were detected at low-level. |
URI: | https://www.sciencedirect.com/science/article/pii/S1876034121003713?via%3Dihub http://hdl.handle.net/123456789/26396 |
ISSN: | 18760341 |
Appears in Collections: | Journal Articles |
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