Please use this identifier to cite or link to this item: http://hdl.handle.net/123456789/22072
Title: Genotoxicity and apoptotic activity of biologically synthesized magnesium oxide nanoparticles against human lung cancer A-549 cell line
Authors: Shahnaz Majeed *1
Mohammed Danish *2
Nur Farisyah Bahriah Binti Muhadi *1
Issue Date: 2018
Publisher: IOP Publishing Ltd
Citation: Majeed, S., Danish, M., & Muhadi, N. F. B. B. (2018). Genotoxicity and apoptotic activity of biologically synthesized magnesium oxide nanoparticles against human lung cancer A-549 cell line. Advances in Natural Sciences: Nanoscience and Nanotechnology, 9(2). https://doi.org/10.1088/2043-6254/aac42c
Abstract: The study focussed on the synthesis of magnesium oxide (MgO) nanoparticles from an aqueous extract of Penicillium species isolated from soil. A suitable amount of magnesium nitrate (MgNO3) was mixed with the aqueous extract of Penicillium. Then the colour of the solution changed due to the formation of MgO nanoparticles. These nascent formed MgO nanoparticles were further confirmed by using UV spectrophotometry which showed the maximum absorption at 215 nm indicating the formation of MgO nanoparticles. Fourier transform infrared spectroscopy (FTIR) was used to find the possible functional groups and proteins involving the stabilization of MgO nanoparticles. Transmission electron microscopy (TEM) study revealed the size, the shape as well as the dispersity of the prepared MgO nanoparticles and showed that they were well dispersed around 12–24 nm (scale 200 nm). The anticancer activity against A-549 cell line of these green synthesized MgO nanoparticles was evaluated. The result showed good anticancer effect after 24 h of incubation. Nevertheless these MgO nanoparticles showed less effect on normal Vero cells. Further apoptotic study clearly displayed the effect of MgO nanoparticles on cancer cells. The effect was observed through chromatin condensation by forming apoptotic bodies using propidium iodide, acridine orange and ethidium bromide (AO/EB) staining technique. The DNA was isolated to confirm the DNA damage; the observation clearly showed DNA damage when compared with DNA ladder.
Description: Author affiliations *1 Faculty of Pharmacy and Health sciences, Universiti Kuala Lumpur, Royal College of Medicine, Perak 30450, Malaysia *2 Bioengineering Technology Section, Universiti Kuala Lumpur Malaysian Institute of Chemical and Bioengineering Technology, Lot 1988, Kawasan Perindustrian Bandar Vendor, 78000, Alor Gajah, Melaka, Malaysia
URI: https://iopscience.iop.org/article/10.1088/2043-6254/aac42c
http://ir.unikl.edu.my/jspui/handle/123456789/22072
ISSN: 2043-6262
Appears in Collections:Journal Articles



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