Abstract:
Response surface methodology was used to
determine optimum conditions for extraction of protein
from fish waste using enzymatic method. The enzymatic
method involved protease enzyme which are alcalase and
protamex. Both methods investigated independent variables
o
C), time (60-240
such as pH (7-11), temperature (40-60
minutes) and ratio of enzyme to sample (0.5-5). Selected
response (dependent variable) which evaluates the
extraction process for both methods were degree of
hydrolysis (%) and protein concentration (mg/mL). From
the results, extraction using enzymatic method-enzyme
alcalase showed the highest yield of protein extracted
(58.37%) and highest protein concentration (7.72 mg/ml)
compared to enzymatic method-enzyme protamex.
