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Title: | Purification of Potent Angiotensin Converting Enzyme (Ace)-Inhibitory Peptides Derived from Red Tilapia (Oreochromis Sp.) by-Products [(Penulenan Peptida Perencat Enzim Penukaran Angiotensin (ACE) daripada Produk Sampingan Ikan Tilapia Merah (Oreochromis Sp.))] |
Authors: | Nur Suraya Abdul Wahab, Emmy Liza Anak Yaji, Norfahana Abd-Talib, Mohammad Zulkeflee Sabri, Kelly Yong, Tau Len and Khairul Faizal Pa’ee (UNIKL MICET) |
Issue Date: | 20-Oct-2023 |
Abstract: | Diet and lifestyle changes are essential alternative treatments for hypertension. Consumers are increasingly more interested in health-promoting ingredients. The use of red tilapia (Oreochromis sp.) by-products (RTBP) has been established as a precursor for protein hydrolysate with angiotensin I-converting (ACE)-inhibitory activity. However, the complexity of the protein hydrolysate reduces its potency. Thus, this work aimed to purify and characterise the ACE-inhibitory hydrolysate derived from red tilapia (Oreochromis sp.). Thermoase PC10F (EC 3.4.24.27) was used in this study to hydrolyse red tilapia by-products (Oreochromis sp.). Meanwhile, ultrafiltration (UF), anion-exchange principle (AEX) and hydrophobic interaction chromatogram (HIC) were applied to purify the hydrolysate. Two molecular weight cut-offs (MWCO) were used for ultrafiltration: 3 kDa and 1 kDa. The 1 kDa hydrolysate showed the highest bioactivity of 85.42% (IC50=0.41 mg mL-). Subsequently, the 1 kDa RTBP hydrolysate was purified based on charge using AEX. Positively charged hydrolysate demonstrated significant bioactivity of 72.32%. In the final purification steps, the hydrophobic fractions showed the highest ACE-inhibitory activity obtained through the hydrophobic interaction chromatogram. The chromatogram yielded two fractions of peaks of high ACE-inhibitory activity on the hydrophobic fraction, which were 90.44% and 95.28%...see more. |
Description: | This article is index by Scopus |
URI: | http://hdl.handle.net/123456789/29089 |
Appears in Collections: | Journal Articles |
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Scopus 2023 Sept_Part5.pdf | 137.21 kB | Adobe PDF | View/Open |
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