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http://hdl.handle.net/123456789/25255
Title: | Electrochemical Immunosensor Based On Highly Sensitive Amino Functionalized Graphene Nanoplatelets-Modified Screen Printed Carbon Electrode |
Authors: | Badruzaman, Nurul Azurin Mohd Azmi, Mohd Azraie Mohd Said, Nur Azura UniKL BMI |
Issue Date: | 26-Apr-2019 |
Publisher: | Scientific.Net |
Citation: | Nurul Azurin Badruzaman, Mohd Azraie Mohd Azmi & Nur Azura Mohd Said. (2020). Electrochemical Immunosensor Based on Highly Sensitive Amino Functionalized Graphene Nanoplatelets-Modified Screen Printed Carbon Electrode. Key Engineering Materials, Volume 833. https://doi.org/10.4028/www.scientific.net/KEM.833.171 |
Abstract: | We presented here the development of an immunosensor based on graphene nanoplatelets-modified screen printed carbon electrode (SPCE) with incorporated rabbit IgG on the amino functionalized surface area. In order to improve sensitivity of working electrode, graphenenanoplatelets solution was fabricated onto surface carbon working electrode. The effect of different (3-aminopropyl) triethoxysilane (APTES) concentrations (0.125, 0.5, 2 and 8% (v/v)) and incubation time of silanization (30, 60 and 90 min) were studied and compared. An electrochemical impedance spectroscopy (EIS) and cyclic voltammetry (CV) were used to characterize our immunosensor based. It is showed that the optimum APTES concentration which provides higher surface coverage and electron transfer rate was 2% concentration (v/v) at 60 min of incubation time. The modified surface was then evaluated by measuring immobilized rabbit IgG via indirect assay using horseradish peroxidase labelled secondary antibody. The optimum detection immobilized IgG was 0.05 mg/mL. These results indicate the potential for amino functionalized graphene nanoplatelets-modified SPCE in detecting protein biomarkers. |
URI: | http://hdl.handle.net/123456789/25255 |
Appears in Collections: | Conference Paper |
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