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metadata.conference.dc.title: Urea Output by L3 Teladorsagia circumcincta and some Properties of Two Urea Producing Enzymes
metadata.conference.dc.contributor.*: Noorzaid Muhamad
LR Walker
DC Simcock
KC Pedley
HV Simpson
S Brown
metadata.conference.dc.subject: Kinetics
Teladorsagia circumcincta
Urea 2013
metadata.conference.dc.publisher: Tehran University of Medical Sciences Publication
metadata.conference.dc.identifier.citation: Iranian Journal of Parasitology 2013. 8(1):137-144
metadata.conference.dc.relation.ispartofseries: Iranian Journal of Parasitology;
metadata.conference.dc.description.abstract: Background: Like several other parasites, Teladorsagia circumcincta secretes or excretes urea, but nei­ther the rate of efflux nor the possible metabolic sources of the urea has been considered. Methods: Parasites were maintained by passage through sheep. Urea efflux was measured using phe­nol/hypochlorite after treatment with urea aminohydrolase. The kinetics of creatine amidinohy­drolase and arginine amidinohydrolase were characterised by coupling the reactions with urea aminohy­drolase and glutamate dehydrogenase. Results: Infective L3 T. circumcincta secreted or excreted urea at 25% of the rate of NH3/NH­4+. The rate of urea efflux was about 84 pmol h‑1 (103 larvae)‑1 over 4 hours, corresponding to about 11 nmol h-1 mg-1 protein. We could not detect urea aminohydrolase activity, but urea production by both creatine amidinohydrolase and arginine amidinohydrolase could be detected. The apparent Km and Vmax of creatine amidinohydrolase were 1.1 mM and 48 nmol h-1 mg-1 protein, respectively, and the activity was greatest at pH 8. The apparent Km and Vmax of arginine amidinohydrolase were 0.7 mM and 62 nmol h-1 mg-1 protein, respectively, and the activity was greatest at pH 7.9. Conclusion: The activity of creatine amidinohydrolase and arginine amidinohydrolase was sufficient to account for the rate of urea secretion or excretion.
metadata.conference.dc.description: Open access journal
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