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Title: In vitro cytotoxic effect of Aspergillus clavatus generated silver nanoparticles on RAW 264.7 cells
Authors: Majeed, Shahnaz
Danish, Mohammed
Mohamad Nasir Mohamad Ibrahim
Siti Hajar Sekeri
Ul Islam, Shah Aarif
Tahir Ansari, Mohammed
Keywords: Aspergillus clavatus
Cytotoxic effect
Transmission electron microscopy
X-ray diffraction
Issue Date: Dec-2020
Publisher: University of Kerbala
Citation: Majeed, S., Danish, M., Mohamad Nasir Mohamad Ibrahim, Siti Hajar Sekeri, Ul Islam, S. A., & Tahir Ansari, M. (2020). In vitro cytotoxic effect of Aspergillus clavatus generated silver nanoparticles on RAW 264.7 cells. Karbala International Journal of Modern Science, 6(4), 344–352.
Abstract: Aspergillus clavatus (A. clavatus ) was utilized for the synthesis of silver nanoparticles (AgNPs) isolated from the soil. . About one (1) mM silver nitrate (AgNO3) was gradually added to the aqueous extract of A. clavatus, the colour of the extract becomes dark brown indicates the AgNPs formation. The UV -Vis spectrophotometry showed the lambda max (-λmax) at 430 nm. Fourier-transform infrared spectroscopy (FTIR) results revealed the peaks at 3221.40 cm N-H symmetric amide, 1645.78 NH bend amine, 1557.02 N-H bend amide groups are associated with AgNPs. The powder form of AgNPs was recorded for X-ray diffraction (XRD) confirms the crystalline nature of AgNPs. Energy dispersive X-rays (EDX) showed the presence of silver, while zeta potential (ζ-potential) -65mV value confirms AgNPs are stable. Transmission electron microscopy (TEM) revealed AgNPs size ranges from 23.65 to 36.99 nm and is spherical in shape, showing no agglomeration. For cytotoxic effect AgNPs showed IC50 value at 125 µg/ ml against RAW cells through MTT assay.
ISSN: 2405609X
Appears in Collections:Journal Articles

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