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Title: | Characterization of Polyphenol Oxidase from Zyzyphus spina-christi from Iraq |
Authors: | S. Al-Jassabi Ali Saad T.R. Satyakeerthy M.S. Abdullah (UniKL RCMP) |
Keywords: | Polyphenol Oxidase Zyzyphus Spina Christi Catechol |
Issue Date: | 2013 |
Publisher: | IDOSI Publications |
Abstract: | Polyphenol oxidase (PPO) from Zyzyphus spina christi found in central part of Iraq was extracted and purified by (NH ) SO precipitation, ion-exchange chromatography and gel filtration chromatography. 42 4 The biochemical characteristics reveal that the PPO from Zyzyphus spina christi has higher affinity towards catechol ( K = 11.4mM and V = 16,400 U/ml min ) at an optimum pH of 5.8. The enzyme had an optimum M max 1 temperature of 37°C and was relatively stable up to 50°C for a period of 60 minutes with almost 80% activity remaining. Among the various PPO inhibitors tested, the most effective inhibitor for the enzyme with 10mM catechol as substrate was ascorbic acid |
Description: | Published in Middle-East Journal of Scientific Research 14 (2): 155-160, 2013. Full text also available at http://www.idosi.org/mejsr/mejsr14%282%2913/2.pdf |
URI: | DOI: 10.5829/idosi.mejsr.2013.14.2.7373 http://localhost/xmlui/handle/123456789/7752 |
ISSN: | Middle-East Journal of Scientific Research |
Appears in Collections: | Journal Articles |
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File | Description | Size | Format | |
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Characterisation of Polyphenol Oxidase from Zyzyphus Spina-Christi from Iraq.pdf | 256.35 kB | Adobe PDF | View/Open |
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