Dielectrophoretic Collection of Lambda-Dna Using Quadruple Electrodes

Abstract

In order to harness the conductivity of Lambda DNA (λ-DNA), the conduction of DNA molecules needs to be determined by DEP trapping experiments. The electrical properties of DNA molecules have been characterized by crossover frequency method. In this work a YOYO®-1 Iodide (purchased from Invitrogen Ltd UK) shows a thousand-fold increase in its green fluorescence when bound to λ-DNA. The double stranded λ-DNA with 48.5 kbp trapped at the high-field region of gold quadruple-electrodes and acting as negative DEP. To ensure the correct system functionalities the simulation of electric fields were performed. The results demonstrate positively behaved particles would be pulled to electrode tips, while negatively behaved particles push away to the regions between the electrodes.