Abstract:
Mannose binding lectin (MBL) is calcium dependent serum protein that used
as a natural immune response and made in the liver. MBL works by binding to
carbohydrates on the surface of pathogens where it can activate the complement
system directly or act as opsonin. The MBL deficiency due to mutation of MBL2 gene
cause people with this mutation has high risk of infection. Low MBL serum levels
will enhance risk of infections whereas high MBL serum levels will increase
inflammatory diseases, transplant rejection and diabetic nephropathy due to its
immune system property. This study suggested an effective purification method to
purify MBL for human based on bovine lung tissue since availability of human
sample was limited. There were two types of column used for purification of MBL
from bovine’s lung tissue in this study which is GlcNAc and mannose-Sepharose
column based on previous study. The protein concentration detect in sample using
Bradford assay show that mannose-Sepharose column can purified MBL better than
GlcNAc column. This study proceeds with SDS-PAGE to prove the present of MBL
protein in bovine’s lung tissue. However there was no band appeared on the gel after
electrophoresis. The factors predicted can caused this mistake were happen during
sample preparation, column preparation, present of detergent, coomassie dye binding
used and method apply during SDS-PAGE.
