Abstract:
Nanotechnology is an emerging field of science and technology at the nanoscale level. The current
study focused on synthesizing copper oxide nanoparticles (CuONPs) from the bacterial cell-free
extract. These copper oxides were further conjugated with polylactic acid. The conjugated CuONPs
(cCuONPs) were characterized by UV-Vis spectrophotometry, FTIR, TGA, SEM, EDX and
TEM.TEM analysis showed the particles are spherical and in the size range 20 – 35 nm. These
cCuONPs showed excellent antibacterial effects against various bacterial pathogens. Furthermore,
cCuONPs exhibit remarkable toxicity towards MDA-MB-231 cells while less toxic towards normal
3T3 cells through MTT assay. Apoptosis study reveals membrane blebbing and chromatin
condensation. cCuONPs showed an excellent release of ROS and arrested the cell cycle at the G2/M
phase. cCuONPs showed mitochondrial membrane depolarisation (Δψm) with 43.61% and extensive
DNA damage on agarose gel electrophoresis. Therefore, these cCuONPs could become the potential
anticancer agent for drug delivery to treat breast cancer and in pharmaceutical and biomedicine
applications.
