Antibacterial Activity of Methanolic Extract of Durio Zibenthinus Murr's Againts E.Coli

Abstract

The study was designed to evaluate antibacterial potential of methanol extract of Durio zibethinus Murr' hulls against Esherichia coli. Extract of the plant was soaked in methanol for one week. then, the methanol extract was filtered using filter paper and was evaporated.The pure filtrates obtained were reconstituted in dimethyl sulfoxide (DMSO) TO MAKE DIFFERENT CONCENTRATIONS ( 100%, 75%, 50% and 25%). The small pieces of sterile diffusion disc was impregnated with each samples of extraction and tested against Escherichia coli using Kirbly Baur technique and the plates were incubated at 37°C. Standard disc of the antibiotic Ciprofloxacin was used as positive control and DMSO was used as negative control. The zone of inhibition was measured after 24 hours and recorded in milimeters. results showed that there was small inhibition zone produced Durio zibethinus murr' hulls methanol extract against E. coli at concentration 100% (4 nmm) and 75% (3 mm). However, there were no inhibition zone observed for 50% and 25% of concentration. As a conclusion, methanol extract of Durio zibethinus murr' hulls exhibit mild synergistic activity against E. coli. Although the effect was but it is possible that Durio zibethinus murr' hulls can be used as antibacterial agent.